Recombinant Prevotella bryantii Glucomannokinase, partial

Product Specifications

Product Name Alternative

Glucomannokinase [Includes: Glucokinase; EC 2.7.1.2) ; Mannokinase; EC 2.7.1.7) ]; Fragment

Abbreviation

Recombinant Prevotella bryantii Glucomannokinase protein, partial

UniProt

P82680

Expression Region

1-25aa

Organism

Prevotella bryantii

Target Sequence

MNEQSMKPYVIGLDLGGTNSVFGIV

Tag

Tag-Free

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

The enzyme has great affinity for glucose and mannose.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

The enzyme has great affinity for glucose and mannose.

Molecular Weight

2.7 kDa

References & Citations

"The glucomannokinase of Prevotella bryantii B14 and its potential role in regulating beta-glucanase expression."Fields M.W., Russell J.B.Microbiology 147:1035-1043 (2001)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.