Recombinant White-rot fungus Cellobiose dehydrogenase (CDH-1), partial
Product Specifications
Product Name Alternative
Cellobiose-quinone oxidoreductase
Abbreviation
Recombinant Phanerochaete chrysosporium CDH-1 protein, partial
Gene Name
CDH-1
UniProt
Q01738
Expression Region
19-208aa
Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Target Sequence
QSASQFTDPTTGFQFTGITDPVHDVTYGFVFPPLATSGAQSTEFIGEVVAPIASKWIGIALGGAMNNDLLLVAWANGNQIVSSTRWATGYVQPTAYTGTATLTTLPETTINSTHWKWVFRCQGCTEWNNGGGIDVTSQGVLAWAFSNVAVDDPSDPQSTFSEHTDFGFFGIDYSTAHSANYQNYLNGDSG
Tag
N-terminal 6xHis-tagged
Type
Developed Protein
Source
Yeast
Field of Research
Microbiology
Relevance
Degrades both lignin and cellulose. Oxidizes cellobiose to cellobionolactone.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Degrades both lignin and cellulose. Oxidizes cellobiose to cellobionolactone.
Molecular Weight
22.3 kDa
References & Citations
"Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants."Li B., Nagalla S.R., Renganathan V.Appl. Environ. Microbiol. 63:796-799 (1997)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Curated Selection
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