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Recombinant Campylobacter jejuni subsp. Major cell-binding factor (peb1A)

Product Specifications

Product Name Alternative

CBF1 PEB1

Abbreviation

Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 peb1A protein

Gene Name

Peb1A

UniProt

A1VZQ4

Expression Region

27-259aa

Organism

Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)

Target Sequence

AEGKLESIKSKGQLIVGVKNDVPHYALLDQATGEIKGFEVDVAKLLAKSILGDDKKIKLVAVNAKTRGPLLDNGSVDAVIATFTITPERKRIYNFSEPYYQDAIGLLVLKEKNYKSLADMKGANIGVAQAATTKKAIGEAAKKIGIDVKFSEFPDYPSIKAALDAKRVDAFSVDKSILLGYVDDKSEILPDSFEPQSYGIVTKKDDPAFAKYVDDFVKEHKNEIDALAKKWGL

Tag

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Microbiology

Relevance

Common antigen and a major cell adherence molecule. Most probably involved, with PEB1C, in a binding-protein-dependent transport system for an amino acid. May be involved in binding to intestinal cells

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Common antigen and a major cell adherence molecule. Most probably involved, with PEB1C, in a binding-protein-dependent transport system for an amino acid. May be involved in binding to intestinal cells.

Molecular Weight

45.4 kDa

References & Citations

"PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in Gram-negative nutrient transport systems."Pei Z., Blaser M.J.J. Biol. Chem. 268:18717-18725 (1993)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Curated Selection

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