Recombinant Erwinia amylovora Major outer membrane lipoprotein (lpp)

Product Specifications

Product Name Alternative

Murein-lipoprotein

Abbreviation

Recombinant Erwinia amylovora lpp protein

Gene Name

Lpp

UniProt

P02939

Expression Region

21-78aa

Organism

Erwinia amylovora

Target Sequence

CSSNAKIDQLSTDVQTLNAKVDQLSNDVTAIRSDVQAAKDDAARANQRLDNQAHSYRK

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Microbiology

Relevance

Interacts with the peptidoglycan both covalently and noncovalently.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Interacts with the peptidoglycan both covalently and noncovalently.

Molecular Weight

22.4 kDa

References & Citations

"Comparison of the lipoprotein gene among the Enterobacteriaceae. DNA sequence of Erwinia amylovora lipoprotein gene."Yamagata H., Nakamura K., Inouye M.J. Biol. Chem. 256:2194-2198 (1981)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.