Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Recombinant Human DNA primase small subunit (PRIM1)

Product Specifications

Product Name Alternative

DNA primase 49KDA subunit Short name: p49

Abbreviation

Recombinant Human PRIM1 protein

Gene Name

PRIM1

UniProt

P49642

Expression Region

1-420aa

Organism

Homo sapiens (Human)

Target Sequence

METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Molecular Weight

65.9 kDa

References & Citations

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features." Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.Mol. Cell. Proteomics 11:M111.015131-M111.015131 (2012)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

KRT76 siRNA (Mouse)
orb1839500-01 15 nmol

KRT76 siRNA (Mouse)

Sign In for Pricing
View Details
KRT76 siRNA (Mouse)
orb1839500-02 30 nmol

KRT76 siRNA (Mouse)

Sign In for Pricing
View Details
Rabbit Anti TNFRSF10D Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot) 200ul
IRBAMLTNFRSF10DAP175200UL 200 µL

Rabbit Anti TNFRSF10D Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot) 200ul

Sign In for Pricing
View Details
CREM (NM_182719) Human Over-expression Lysate
LH16971V 100 µg

CREM (NM_182719) Human Over-expression Lysate

Sign In for Pricing
View Details
Anti-SEC24C antibody
STJ112693 100 µl

Anti-SEC24C antibody

Sign In for Pricing
View Details
Ovary specific acidic protein (MGARP) (NM_032623) Human Tagged ORF Clone Lentiviral Particle
RC216908L4V 200 µL

Ovary specific acidic protein (MGARP) (NM_032623) Human Tagged ORF Clone Lentiviral Particle

Sign In for Pricing
View Details