Recombinant Dictyostelium discoideum Ponticulin (ponA)

Product Specifications

Product Name Alternative

PonA; DDB_G0293522; Ponticulin

Abbreviation

Recombinant Dictyostelium discoideum ponA protein

Gene Name

PonA

UniProt

P54660

Expression Region

23-118aa

Organism

Dictyostelium discoideum (Slime mold)

Target Sequence

QYTLSVSNSASGSKCTTAVSAKLNACNTGCLNSFNIVESSNGKGLVFKTFINAACSGEYESLSQFTCAANQKIPTTSYIVSCNSTPSSNSTTDSDS

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Microbiology

Relevance

Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network.

Molecular Weight

13.9 kDa

References & Citations

"Identification of a second member of the ponticulin gene family and its differential expression pattern."Hitt A.L., Iijima-Shimizu M., DuBay M.J., Antonette L.L., Urushihara H., Wilkerson C.G.Biochim. Biophys. Acta 1628:79-87 (2003) .

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.