Recombinant Bovine Galectin-1 (LGALS1)

Product Specifications

Product Name Alternative

14KDA lectin Beta-galactoside-binding lectin L-14-I Galaptin Lactose-binding lectin 1 Lectin galactoside-binding soluble 1 S-Lac lectin 1

Abbreviation

Recombinant Bovine LGALS1 protein

Gene Name

LGALS1

UniProt

P11116

Expression Region

2-135aa

Organism

Bos taurus (Bovine)

Target Sequence

ACGLVASNLNLKPGECLRVRGEVAADAKSFLLNLGKDDNNLCLHFNPRFNAHGDVNTIVCNSKDAGAWGAEQRESAFPFQPGSVVEVCISFNQTDLTIKLPDGYEFKFPNRLNLEAINYLSAGGDFKIKCVAFE

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Cell Biology

Relevance

Lectin that binds beta-galactoside and a wide array of complex carbohydrates (PubMed:1900835, PubMed:8108426, PubMed:7773775) . Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Lectin that binds beta-galactoside and a wide array of complex carbohydrates

Molecular Weight

30.6 kDa

References & Citations

"Soluble bovine galactose-binding lectin. cDNA cloning reveals the complete amino acid sequence and an antigenic relationship with the major encephalitogenic domain of myelin basic protein."Abbott W.M., Mellor A., Edwards Y., Feizi T.Biochem. J. 259:283-290 (1989)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.