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Recombinant Human Phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1)

Product Specifications

Product Name Alternative

PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase

Abbreviation

Recombinant Human PHOSPHO1 protein

Gene Name

PHOSPHO1

UniProt

Q8TCT1

Expression Region

1-267aa

Organism

Homo sapiens (Human)

Target Sequence

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Signal Transduction

Relevance

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . Involved in the generation of inorganic phosphate for bone mineralization.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . Involved in the generation of inorganic phosphate for bone mineralization.

Molecular Weight

33.7 kDa

References & Citations

"Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities."Roberts S.J., Stewart A.J., Sadler P.J., Farquharson C.Biochem. J. 382:59-65 (2004)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

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