Recombinant Entamoeba histolytica Calreticulin
Product Specifications
Product Name Alternative
Calreticulin; Fragment
Abbreviation
Recombinant Entamoeba histolytica Calreticulin protein
UniProt
P83003
Expression Region
1-19aa
Organism
Entamoeba histolytica
Target Sequence
KVYFEETFENGWKRIWSKW
Tag
N-terminal GST-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Tags & Cell Markers
Relevance
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity) .
Molecular Weight
29.5 kDa
References & Citations
"Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (SwissProt: accession P83003) ."Gonzalez E., Rico G., Mendoza G., Ramos F., Garcia G., Moran P., Valadez A., Melendro E.I., Ximenez C.Am. J. Trop. Med. Hyg. 67:636-639 (2002)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Curated Selection
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