Recombinant Mouse All-trans-retinol dehydrogenase [NAD (+) ] ADH7 (Adh7)

Product Specifications

Product Name Alternative

ADH-C2 Alcohol dehydrogenase 7 Alcohol dehydrogenase class IV mu/sigma chain Gastric alcohol dehydrogenase Retinol dehydrogenase

Abbreviation

Recombinant Mouse Adh7 protein

Gene Name

Adh7

UniProt

Q64437

Expression Region

1-374aa

Organism

Mus musculus (Mouse)

Target Sequence

MGTAGKVIKCKAAVLWGVNQPFSIEEIEVAPPKAKEVRVKILATGICRTDDHIIKGSMVSKFPVIVGHEAVGVVESVGEGVTTVRPGDKVIPLFLPQCRECNACLNPEGNLCIRSDLTGRGVLADGTTRFTCKGKPVQHFMNTSTFTEYTVLDESSVAKVDGAAPPEKACLIGCGFSTGYGAAVKTAKVTPGSTCVVFGLGGVGLSVIMGCKAAGASRIIGIDINKDKFQKALAVGATECISPKDSTKPISEVLSDMTGNTIQYTFEVIGRLETMVDALSSCHMNYGTSVVVGAPPSAKMLTYDPMLLFTGRTWKGCVFGGWKSRDDVPKLVTEFLEKKFDLDQLITHTLPFNNINEGFELLYSGKSIRTVLTF

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Metabolism

Relevance

Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation.

Molecular Weight

55.9 kDa

References & Citations

"Cloning of the mouse class IV alcohol dehydrogenase (retinol dehydrogenase) cDNA and tissue-specific expression patterns of the murine ADH gene family."Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G.J. Biol. Chem. 270:10868-10877 (1995)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.