Recombinant Dog Arginine esterase

Product Specifications

Product Name Alternative

Arginine esterase; EC 3.4.21.35

Abbreviation

Recombinant Dog Arginine esterase protein

UniProt

P09582

Expression Region

25-260aa

Organism

Canis lupus familiaris (Dog) (Canis familiaris)

Target Sequence

IIGGRECLKNSQPWQVAVYHNGEFACGGVLVNPEWVLTAAHCANSNCEVWLGRHNLSESEDEGQLVQVRKSFIHPLYKTKVPRAVIRPGEDRSHDLMLLHLEEPAKITKAVRVMDLPKKEPPLGSTCYVSGWGSTDPETIFHPGSLQCVDLKLLSNNQCAKVYTQKVTKFMLCAGVLEGKKDTCKGDSGGPLICDGELVGITSWGATPCGKPQMPSLYTRVMPHLMWIKDTMKANT

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

This serine protease is found in dog seminal plasma, its exact physiological function is not known. Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

This serine protease is found in dog seminal plasma, its exact physiological function is not known.

Molecular Weight

42 kDa

References & Citations

"Nucleotide sequence of the androgen-dependent arginine esterase mRNA of canine prostate."Chapdelaine P., Ho-Kim M.-A., Tremblay R.R., Dube J.Y.FEBS Lett. 232:187-192 (1988)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.