Recombinant Dermatophagoides farinae Mite group 2 allergen Der f 2 (DERF2)
Product Specifications
Product Name Alternative
Allergen Der f II Allergen: Der f 2
Abbreviation
Recombinant Dermatophagoides farinae DERF2 protein
Gene Name
DERF2
UniProt
Q00855
Expression Region
18-146aa
Organism
Dermatophagoides farinae (American house dust mite)
Target Sequence
DQVDVKDCANNEIKKVMVDGCHGSDPCIIHRGKPFTLEALFDANQNTKTAKIEIKASLDGLEIDVPGIDTNACHFMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKLIGDNGVLACAIATHGKIRD
Tag
N-terminal 6xHis-tagged
Type
In Stock Protein
Source
Yeast
Field of Research
Others
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
16.1 kDa
References & Citations
"Cloning and expression of cDNA coding for the major house dust mite allergen Der f II in Escherichia coli."Yuuki T., Okumura Y., Ando T., Yamakawa H., Suko M., Haida M., Okudaira H.Agric. Biol. Chem. 55:1233-1238 (1991)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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