Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

Product Specifications

Product Name Alternative

Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor (CSF) ; Molgramostin; Sargramostim

Abbreviation

Recombinant Human CSF2 protein (Active)

Gene Name

CSF2

UniProt

P04141

Expression Region

18-144aa

Organism

Homo sapiens (Human)

Target Sequence

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Tag

N-terminal 6xHis-tagged

Type

Active Protein & In Stock Protein

Source

Yeast

Field of Research

Immunology

Relevance

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 21.07-35.48 pg/mL.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Molecular Weight

17.1 kDa

References & Citations

GM-CSF Primes Proinflammatory Monocyte Responses in Ankylosing Spondylitis. Shi H., Chen L., Ridley A., Zaarour N., Brough I., Caucci C., Smith J.E., Bowness P. Front Immunol 11:1520-1520 (2020)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.