Recombinant Dermatophagoides pteronyssinus Peptidase 1 (DERP1)

Product Specifications

Product Name Alternative

Allergen Der p I Major mite fecal allergen Der p 1 Allergen: Der p 1

Abbreviation

Recombinant Dermatophagoides pteronyssinus DERP1 protein

Gene Name

DERP1

UniProt

P08176

Expression Region

99-320aa

Organism

Dermatophagoides pteronyssinus (European house dust mite)

Target Sequence

TNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQHNGVVQESYYRYVAREQSCRRPNAQRFGISNYCQIYPPNVNKIREALAQTHSAIAVIIGIKDLDAFRHYDGRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.

Molecular Weight

38.0 kDa

References & Citations

"Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli."Thomas W.R., Stewart G.A., Simpson R.J., Chua K.Y., Plozza T.M., Dilworth R.J., Nisbet A., Turner K.J.Int. Arch. Allergy Appl. Immunol. 85:127-129 (1988)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.