Recombinant Bacillus subtilis Subtilisin E (aprE)
Product Specifications
Product Name Alternative
AprE; apr; aprA; sprE; BSU10300; Subtilisin E; EC 3.4.21.62
Abbreviation
Recombinant Bacillus subtilis aprE protein
Gene Name
AprE
UniProt
P04189
Expression Region
107-381aa
Organism
Bacillus subtilis (strain 168)
Target Sequence
AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
Tag
N-terminal 6xHis-SUMO-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Subtilisin is an Extracellular domain alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid
Buffer
Tris/PBS-based buffer, 5%-50% glycerol
Function
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Molecular Weight
43.7 kDa
References & Citations
"Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation."Stahl M.L., Ferrari E.J. Bacteriol. 158:411-418 (1984)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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