Recombinant Sorangium cellulosum Mannonate dehydratase (uxuA)

Product Specifications

Product Name Alternative

D-mannonate hydro-lyase

Abbreviation

Recombinant Sorangium cellulosum uxuA protein

Gene Name

UxuA

UniProt

A9EVN4

Expression Region

1-357aa

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56) )

Target Sequence

MKMTFRWYGENDPVKLEYIRQIPGIYGIVSAIYDVPVGEVWPVERLQALRARVEGVGLRFEVVESVPVHEDIKLGKPARERLIDNFCQNIRNCAAAGVKVICYNFMPVFDWTRTEMARELPDGSLTLAFDAAAVAEIDPEQGISLPGWDSSYRPEQLRAVLDEYKNVSEEALWEHLEHFLRRIIPVAEEVGVRMAMHPDDPPRPIFGLPRIVKNRDDLMRLVKTVDSPANGITLCSGSLGADLCNNIVSLVREFGGMGRIPFGHLRNVAVQEDGTFFESAHLSCEGSLDMAAIVKAYHDVGFDGYVRPDHGRMIWGETGKPGYGLYDRALGLVYLNGLWEATCKLSPGPAAKVPAPG

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Catalyzes the dehydration of D-mannonate.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Catalyzes the dehydration of D-mannonate.

Molecular Weight

55.7 kDa

References & Citations

"Complete genome sequence of the myxobacterium Sorangium cellulosum."Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. Mueller R.Nat. Biotechnol. 25:1281-1289 (2007)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.