Recombinant Rat Trophoblast glycoprotein (Tpbg), partial

Product Specifications

Product Name Alternative

5T4 oncofetal trophoblast glycoprotein Short name: 5T4 oncotrophoblast glycoprotein Wnt-activated inhibitory factor 1 Short name: WAIF1

Abbreviation

Recombinant Rat Tpbg protein, partial

Gene Name

Tpbg

UniProt

Q5PQV5

Expression Region

32-361aa

Organism

Rattus norvegicus (Rat)

Target Sequence

SAPSSSLPSSSTSPAAFLASGSAQPPPAERCPAACECSEAARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLAVLNLSGNHLKEVGAGAFEHLPGLRRLDLSHNPLTNLSAFTFAGSNVSVSTPSPLLELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALRSGLALRGLHHLELASNHFLYLPRDLLDQLPSLKHLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLHNSTLAEWQGLAHVRVFLDNNPWVCDCYMADMVSWLKETEVVPDKARLTCAFPEKMRNRGLLDLTSSDLDCDATLPQSLQTS

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Molecular Weight

51.8 kDa

References & Citations

"Structure and expression of the rat 5T4 gene."Ninkina N.N., Buchman V.L.Submitted (MAY-1998)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.