Recombinant Geobacillus stearothermophilus Glycerol-1-phosphate dehydrogenase [NAD (P) +] (egsA)

Product Specifications

Product Name Alternative

Enantiomeric glycerophosphate synthase sn-glycerol-1-phosphate dehydrogenase

Abbreviation

Recombinant Geobacillus stearothermophilus egsA protein

Gene Name

EgsA

UniProt

B3EYN8

Expression Region

1-404aa

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Target Sequence

MNQTLEEVVSLARQCGCGHRHYDIPIEQMVVGREAFARLVAYLRHKRYERVAIVADDHTFAAVERSLCDQLENGSIRYTVCLVQPDENGDVIADERSIVQVLLETPDDVDVLIAVGAGTIHDITRFSSYKMRIPFISVPTAPSVDGFTSMGAPLIIRGVKKTIQAQAPIAVFAHTGVLCQSPKEMIAAGFGDMVAKYTSLADWQFAHWMADEPYCPFVHQLTEQSLQTCVDHIDDIAAGGEQGIRVLMDALLQSGIAMLLMGQSYSASGAEHHLSHYWEMEFLRQKKRQVLHGAKVGVSTPIIIEHYQRVFWPLLNELEKRPKSMDEATWERLKANTASIRELLESLPSPERIRTMLAKVGGAIAPEQLGIDPQLVERSLREAHRLRLNRFTMLYFLNELIFVE

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Catalyzes the NAD (P) H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P) . The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Molecular Weight

61.4 kDa

References & Citations

"Hemicellulose utilization cluster in Geobacillus stearothermophilus strain T-6."Shoham Y., Shulami S., Ben-David A., Langut Y.Submitted (JUN-2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.