Recombinant Human Alcohol dehydrogenase class-3 (ADH5)

Product Specifications

Product Name Alternative

Alcohol dehydrogenase 5Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-IIIGlutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-) ; FALDH ; FDH ; GSH-FDHS- (hydroxymethyl) glutathione dehydrogenase (EC:1.1.1.284)

Abbreviation

Recombinant Human ADH5 protein

Gene Name

ADH5

UniProt

P11766

Expression Region

2-374aa

Organism

Homo sapiens (Human)

Target Sequence

ANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Metabolism

Relevance

Class-III ADH is rarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S- (hydroxymethyl) glutathione.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S- (hydroxymethyl) glutathione.

Molecular Weight

41.6 kDa

References & Citations

CDNA sequence of human class III alcohol dehydrogenase.Sharma C.P., Fox E.A., Holmquist B., Joernvall H., Vallee B.L.Biochem. Biophys. Res. Commun. 164:631-637 (1989)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.