Recombinant Escherichia coli GTP cyclohydrolase-2 (ribA)

Product Specifications

Product Name Alternative

GTP cyclohydrolase II

Abbreviation

Recombinant E.coli ribA protein

Gene Name

RibA

UniProt

P0A7I7

Expression Region

1-196aa

Organism

Escherichia coli (strain K12)

Target Sequence

MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4 (3H) -pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4 (3H) -pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.

Molecular Weight

25.8 kDa

References & Citations

Biosynthesis of riboflavin studies on the mechanism of GTP cyclohydrolase II.Ritz H., Schramek N., Bracher A., Herz S., Eisenreich W., Richter G., Bacher A.J. Biol. Chem. 276:22273-22277 (2001)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.