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Recombinant Arabidopsis thaliana Putative protease Do-like 12, mitochondrial (DEGP12)

Product Specifications

Product Name Alternative

DEGP12; At3g16550; MDC8.18Putative protease Do-like 12; mitochondrial; EC 3.4.21.-

Abbreviation

Recombinant Mouse-ear cress DEGP12 protein

Gene Name

DEGP12

UniProt

Q9LK70

Expression Region

25-499aa

Organism

Arabidopsis thaliana (Mouse-ear cress)

Target Sequence

SRIATIVLPFALTRGRKIHTMSKDEEWWKKIRKSPPVDELMLESVVEVFTDSTKYSKVKPWQTLNQESYGGSGFAIAGKKILTNAHVVEGMNDHIFVHVKRHGSQVKYKAKVQKIAHECDLAILEIDSDEFWKGMNPLEFGDIPPLNEIVYVVGYPKAGETICVTKGVVTGVKTGNYLRSSTKLLTIHIDATTYGGNSGGPVITGDKVLGVLFQILGDKKSTGVVIPTPIIRHFITGAEESSHNAVFGSLVLSCQSMKNAQIRNHFKMSPETTGILINKINSSSGAHKILRKDDIILAIDGVPVLSEMRRISFNHFISMKKPDENILVKVLRKGKEHEYNISLKPVKPHIQVQQYYNLPSYYIFGGFVFVPLTKSYIDDKYYKITDEQHVIISQVMPDDINKGYSNFKDLQVEKVNGVKVKNLKHLRELIEGCFSKDLRLDLENDKVMVLNYESAKKATFEILERHNIKSAWASE

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Putative serine protease.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Putative serine protease.

Molecular Weight

69.5 kDa

References & Citations

"Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature."Adam Z., Adamska I., Nakabayashi K., Ostersetzer O., Haussuhl K., Manuell A., Zheng B., Vallon O., Rodermel S.R., Shinozaki K., Clarke A.K.Plant Physiol. 125:1912-1918 (2001)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Frequently Asked Questions

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