Recombinant Human PDZ and LIM domain protein 3 (PDLIM3)
Product Specifications
Product Name Alternative
Actinin-associated LIM protein; Alpha-actinin-2-associated LIM protein
Abbreviation
Recombinant Human PDLIM3 protein
Gene Name
PDLIM3
UniProt
Q53GG5
Expression Region
1-189aa
Organism
Homo sapiens (Human)
Target Sequence
MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQKTGTTLNTSIIFGPNLS
Tag
N-terminal 6xHis-SUMO-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Signal Transduction
Relevance
May play a role in the organization of actin filament arrays within muscle cells.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
May play a role in the organization of actin filament arrays within muscle cells.
Molecular Weight
36.2 kDa
References & Citations
Actinin-associated LIM protein identification of a domain interaction between PDZ and spectrin-like repeat motifs.Xia H., Winokur S.T., Kuo W.-L., Altherr M.R., Bredt D.S.J. Cell Biol. 139:507-515 (1997)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Isoform 3
Available Sizes
Frequently Asked Questions
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