Recombinant Human PDZ and LIM domain protein 3 (PDLIM3)

Product Specifications

Product Name Alternative

Actinin-associated LIM protein; Alpha-actinin-2-associated LIM protein

Abbreviation

Recombinant Human PDLIM3 protein

Gene Name

PDLIM3

UniProt

Q53GG5

Expression Region

1-189aa

Organism

Homo sapiens (Human)

Target Sequence

MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQKTGTTLNTSIIFGPNLS

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Signal Transduction

Relevance

May play a role in the organization of actin filament arrays within muscle cells.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

May play a role in the organization of actin filament arrays within muscle cells.

Molecular Weight

36.2 kDa

References & Citations

Actinin-associated LIM protein identification of a domain interaction between PDZ and spectrin-like repeat motifs.Xia H., Winokur S.T., Kuo W.-L., Altherr M.R., Bredt D.S.J. Cell Biol. 139:507-515 (1997)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.