Recombinant Staphylococcus aureus Transposase for transposon Tn552
Product Specifications
Product Name Alternative
Transposase for transposon Tn552; ORF 480
Abbreviation
Recombinant Staphylococcus aureus Tn552 protein
Gene Name
Tn552
UniProt
P18416
Expression Region
1-480aa
Organism
Staphylococcus aureus
Target Sequence
MKNKEKYLTNFSEAKRKEATQKYNIIKPFILGKQSLSSISKSKGIALSTLYRWNKLYKEQGLTGLIHNTRVDKGEHKLKQNIIDEIKRLALKNKRNSIATIHRKIANYCIENNFYKPSYKQVYSIIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPNEIWQADHTLLDIYILDQKGNINRPWLTIIMDDYSRAIAGYFISFDAPNAQNTALTLHQAIWNKNNTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIERFFQTVNQTFLEQLPGYINNNDTSSDLIDFQNFEEKLRYFLIEDYNQKEHSAIQSTPINRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKIHSDGIHFQGFRYSNTNLTAYVGEYVLIRYNPNDMAEIRVFYRDEFLCTAISPDLADYSIDIKEIQHARSQRRKHLKQNIASPSTTDLIKEEKSYGYSPQETTKNVKKLKRYRND
Tag
N-terminal 6xHis-SUMO-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
72.3 kDa
References & Citations
Tn552, a novel transposable element from Staphylococcus aureus.Rowland S.J., Dyke K.G.H.Mol. Microbiol. 4:961-975 (1990)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Curated Selection
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