Recombinant Chicken Vitellogenin-1 (VTG1), partial

Product Specifications

Product Name Alternative

Minor vitellogeninVitellogenin I

Abbreviation

Recombinant Chicken VTG1 protein, partial

Gene Name

VTG1

UniProt

P87498

Expression Region

24-663aa

Organism

Gallus gallus (Chicken)

Target Sequence

FGENKVYTYNYESILFSGIPEKGLARTGIRIRSEVEISGIGPKLCLIRIHSIEAAEYNGIWPTSSFSRSLKLTQALTGQLSIPIKFEYSNGHVGNLMAPDSVSDDGLNIYRGILNILELSLKKMQHSYSIQEAGIGGICNTTYAIQENKKANLVDVTKSKDLNSCEEKVQVVTGSAYTQPCQTCQQRNKNSRATATYNYKIKYTHNEAVITQAEVEEVHQFTPFHEITGGNAIVEARQKLALIEVQKQVAEVPPKEFQKRGSLQYQFGSELLQLPVHLFKIKDVERQIEERLQDLVETTYEQLPSDAPAKALKLMHLLRAANEENYESVWKQFSSRPAYRRYLLDLLPAAASHRSLRFLRHKMERQELTNWEIAQTVLVALHSSSPTQEVMEEATLIVKKHCPRSSSVLRKVCLLSYASLCHKRCSSPYSCSECLQVFHVFAGEALGKSNIEEVLLALKALGNVGHPASIKHIKKFLPGYAAGASELPLKVHETAVMALKSIGMRDPQMVQAITLEIFLNHKIHPRIRMLAAVVLLETKPGLPILMILVDAVLKEPSMQVASFIYSHLRALGRSTAPDLQMMASACRMAVRALSPKFDRSGYQFSKVFRFSMFKEFLMSGLAAKYFVLNNAGSLIPTMAV

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Precursor of the egg-yolk proteins that are sources of nutrients during early development of oviparous organisms.Phosvitin is believed to be of importance in sequestering calcium, iron and other cations for the developing bryo.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Precursor of the egg-yolk proteins that are sources of nutrients during early development of oviparous organisms.; FUNCTION

Molecular Weight

75.6 kDa

References & Citations

Precursor-product relationship between chicken vitellogenin and the yolk proteins the 40KDA yolk plasma glycoprotein is derived from the C-terminal cysteine-rich domain of vitellogenin II.Yamamura J., Adachi T., Aoki N., Nakajima H., Nakamura R., Matsuda T.Biochim. Biophys. Acta 1244:384-394 (1995)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.