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Recombinant Human TATA box-binding protein-like protein 1 (TBPL1), partial

Product Specifications

Product Name Alternative

21KDA TBP-like protein; Second TBP of unique DNA protein ; STUDTATA box-binding protein-related factor 2 ; TBP-related factor 2TBP-like factorTBP-related protein

Abbreviation

Recombinant Human TBPL1 protein, partial

Gene Name

TBPL1

UniProt

P62380

Expression Region

1-185aa

Organism

Homo sapiens (Human)

Target Sequence

MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEI

Tag

N-terminal 6xHis-SUMO-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Epigenetics and Nuclear Signaling

Relevance

Part of a specialized transcription syst that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter elent at these genes. Ses to also mediate the transcription of NF1. Does not bind the TATA box.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.

Molecular Weight

36.8 kDa

References & Citations

TRF2, but not TBP, mediates the transcription of ribosomal protein genes.Wang Y.L., Duttke S.H., Chen K., Johnston J., Kassavetis G.A., Zeitlinger J., Kadonaga J.T.Genes Dev. 28:1550-1555 (2014)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

Curated Selection

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