Recombinant Human TATA box-binding protein-like protein 1 (TBPL1), partial
Product Specifications
Product Name Alternative
21KDA TBP-like protein; Second TBP of unique DNA protein ; STUDTATA box-binding protein-related factor 2 ; TBP-related factor 2TBP-like factorTBP-related protein
Abbreviation
Recombinant Human TBPL1 protein, partial
Gene Name
TBPL1
UniProt
P62380
Expression Region
1-185aa
Organism
Homo sapiens (Human)
Target Sequence
MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEI
Tag
N-terminal 6xHis-SUMO-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Epigenetics and Nuclear Signaling
Relevance
Part of a specialized transcription syst that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter elent at these genes. Ses to also mediate the transcription of NF1. Does not bind the TATA box.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.
Molecular Weight
36.8 kDa
References & Citations
TRF2, but not TBP, mediates the transcription of ribosomal protein genes.Wang Y.L., Duttke S.H., Chen K., Johnston J., Kassavetis G.A., Zeitlinger J., Kadonaga J.T.Genes Dev. 28:1550-1555 (2014)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Curated Selection
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