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Recombinant Mycoplasma hyorhinis Variant surface antigen F (vlpF), partial

Product Specifications

Product Name Alternative

(VlpF prolipoprotein)

Abbreviation

Recombinant Mycoplasma hyorhinis vlpF protein, partial

Gene Name

VlpF

UniProt

Q49538

Expression Region

30-67aa

Organism

Mycoplasma hyorhinis

Target Sequence

CGQTNTDQSQQPGSGSGSGSGTSNGSGSTPTPEQGNNQ

Tag

N-terminal 6xHis-KSI-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Responsible for the antigenic diversity for host adaptation. Expression in E.coli of a construct containing vlpD, vlpE, and vlpF yields antigenically distinguishable products corresponding to each gene.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

18.9 kDa

References & Citations

"Increased structural and combinatorial diversity in an extended family of genes encoding Vlp surface proteins of Mycoplasma hyorhinis." Yogev D., Watson-Mckown R., Rosengarten R., Im J., Wise K.S. J. Bacteriol. 177:5636-5643 (1995)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

Curated Selection

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