Recombinant Mycoplasma hyorhinis Variant surface antigen F (vlpF), partial
Product Specifications
Product Name Alternative
(VlpF prolipoprotein)
Abbreviation
Recombinant Mycoplasma hyorhinis vlpF protein, partial
Gene Name
VlpF
UniProt
Q49538
Expression Region
30-67aa
Organism
Mycoplasma hyorhinis
Target Sequence
CGQTNTDQSQQPGSGSGSGSGTSNGSGSTPTPEQGNNQ
Tag
N-terminal 6xHis-KSI-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Responsible for the antigenic diversity for host adaptation. Expression in E.coli of a construct containing vlpD, vlpE, and vlpF yields antigenically distinguishable products corresponding to each gene.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
18.9 kDa
References & Citations
"Increased structural and combinatorial diversity in an extended family of genes encoding Vlp surface proteins of Mycoplasma hyorhinis." Yogev D., Watson-Mckown R., Rosengarten R., Im J., Wise K.S. J. Bacteriol. 177:5636-5643 (1995)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Curated Selection
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