Rhodamine 123

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

Product Specifications

CAS Number

[62669-70-9]

Product Name Alternative

RH-123; R-22420

UNSPSC

12171500

Hazard Statement

H302

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Applications

COVID-19-immunoregulation

Field of Research

Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/Rhodamine_123.html

Concentration

10mM

Purity

99.73

Solubility

DMSO : 41.67 mg/mL (ultrasonic)

Smiles

O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]

Molecular Formula

C21H17ClN2O3

Molecular Weight

380.82

Precautions

H302

References & Citations

[1]M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35 (7) : 1276–1285.|[2]Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986) . Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850 (3), 436–448.