Anti-HIF1 α Antibody

Mouse Monoclonal Antibody specific to HIF1 α

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HIF-1-α, HIF1-α, ARNT-interacting protein

Gene Name

Hif1a

NCBI Gene ID

UniProt

Q61221

Cellular Locus

Cytoplasm, Nucleus, Nucleus speckle

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein corresponding to aa 329-530 of human HIF-1a.

Target Antigen

Hypoxia-inducible factor 1-α

Target

HIF1 α

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, ELISA

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity). {UniProtKB:Q16665, PubMed:15225651, PubMed:17981124, PubMed:22009797, PubMed:26245371}.

Additionnal Information

Immunoblotting: use at 2ug/mL. Predicted molecular weight ~116kDa.This is a recommended concentration. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, and bovine HIF-1alpha.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor that regulates the transcription of a broad range of genes that facilitate responses to hypoxia including genes that regulate angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. HIF-1 is comprised of two subunits, HIF-1alpha and HIF-1beta. The widely expressed HIF-1alpha is degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH)-E3 ligase complex; ubiquitination and proteasomal degradation follows.