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LIG1/DNA ligase I Antibody

Goat polyclonal antibody to LIG1

Product Specifications

Product Name Alternative

Anti LIG1 antibody, anti ligase I, DNA, ATP-dependent antibody, anti MGC117397 antibody, anti MGC130025 antibody, anti DNA ligase I antibody

Reactivity

Human

Target

LIG1 / DNA ligase I

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RVREDKQPEQATTS

Field of Research

Molecular Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.1-0.3 μg/ml

Molecular Weight

102

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml.

Prediction Reactivity

Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

3978

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