LIG1/DNA ligase I Antibody
Goat polyclonal antibody to LIG1
Product Specifications
Product Name Alternative
Anti LIG1 antibody, anti ligase I, DNA, ATP-dependent antibody, anti MGC117397 antibody, anti MGC130025 antibody, anti DNA ligase I antibody
Reactivity
Human
Target
LIG1 / DNA ligase I
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RVREDKQPEQATTS
Field of Research
Molecular Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.1-0.3 μg/ml
Molecular Weight
102
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml.
Prediction Reactivity
Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
3978
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