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Goat anti-C14orf68 (aa242-254) Antibody

Goat polyclonal antibody to C14orf68

Product Specifications

Product Name Alternative

Anti C14orf68 antibody, anti chromosome 14 open reading frame 68 antibody, anti HDMCP antibody, anti HMFN1655 antibody, anti hepatocellular carcinoma-downregulated mitochondrial carrier protein antibody

Reactivity

Human

Target

C14orf68 (aa242-254)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KSRLQADGQGQRR

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5 μg/ml, IHC-P: 2.5μg/ml

Molecular Weight

33.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

283600

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