Goat anti-C14orf68 (aa242-254) Antibody
Goat polyclonal antibody to C14orf68
Product Specifications
Product Name Alternative
Anti C14orf68 antibody, anti chromosome 14 open reading frame 68 antibody, anti HDMCP antibody, anti HMFN1655 antibody, anti hepatocellular carcinoma-downregulated mitochondrial carrier protein antibody
Reactivity
Human
Target
C14orf68 (aa242-254)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KSRLQADGQGQRR
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5 μg/ml, IHC-P: 2.5μg/ml
Molecular Weight
33.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
283600
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