Q1: What is Recombinant Human RM03 (His)?
A: Recombinant Human RM03 (His) is a high-purity human ribosomal protein expressed in E. coli and supplied with a His-tag for easy purification and detection. It is intended for research use only.
Q2: What is the His-tag for?
A: The His-tag is a short chain of histidine residues attached to the protein. It allows efficient purification using nickel or cobalt affinity resins and simplifies detection in assays like Western blot and ELISA.
Q3: What applications is RM03 (His) suitable for?
A: This protein is commonly used in ELISA, Western blotting, SDS-PAGE analysis, protein interaction studies, and as a standard or control in research experiments.
Q4: How should I store RM03 (His)?
A: Store the protein at −20 °C or below. Avoid repeated freeze-thaw cycles to maintain stability. Lyophilized forms should be reconstituted according to the datasheet.
Q5: Can RM03 (His) be used for therapeutic purposes?
A: No, this product is strictly for research use only and is not approved for therapeutic or diagnostic applications.
Q6: How pure is Recombinant Human RM03 (His)?
A: RM03 (His) is provided at high purity suitable for most laboratory assays. Purity can be verified using SDS-PAGE or Western blot as recommended in the datasheet.
Q7: Can the His-tag be removed?
A: In some cases, if a protease cleavage site is included in the construct, the His-tag can be removed enzymatically. Check the datasheet or contact the supplier for details.
Q8: How is Recombinant Human RM03 (His) supplied?
A: It is supplied as a filtered protein solution in PBS with glycerol or as a lyophilized powder, depending on the product variant.