DOTAP (methylsulfate)

DOTAP methylsulfat is a cationic lipid reagent, a cationic derivative of trimethylammonium, linked to two 18-carbon fatty acid tails, each with a single olefin group. DOTAP methylsulfat can self-assemble with negatively charged ions (such as DNA) to form complexes, which can be adsorbed to the cell membrane surface and enter the cell by electrostatic interaction and endocytosis, respectively. DOTAP methylsulfat promotes endosomal membrane fusion with its own hydrophobic domain, releases DNA into the cytoplasm, and exerts gene delivery function. DOTAP methylsulfat can be widely used in research fields such as gene therapy, cell transfection, and non-viral vector design[1][2].

Product Specifications

CAS Number

[144189-73-1]

Product Name Alternative

1,2-Dioleoyl-3-trimethylammonium-propane (methylsulfate)

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H331, H336, H351, H361, H372

Target

Liposome

Type

Biochemical Assay Reagents

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/dotap-methylsulfate.html

Purity

99.94

Solubility

DMSO : 5 mg/mL (ultrasonic) |Ethanol : 100 mg/mL

Smiles

S(OC)(=O)(=O)[O-].C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C

Molecular Formula

C43H83NO8S

Molecular Weight

774.19

Precautions

H302, H315, H319, H331, H336, H351, H361, H372

References & Citations

[1]Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim Biophys Acta. 2015 Oct;1848 (10 Pt A) :1996-2001.|[2]Crook K, et al. Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther. 1998 Jan;5 (1) :137-43.