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Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (MYH11/2303R), Biotin conjugate

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

AAT4; Myosin heavy chain 11 (MYH11); Myosin heavy chain 11 smooth muscle; Smooth muscle myosin heavy chain 11; Myosin-11; SM1; SM2; SMHC; SMMHC

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MYH11

Gene ID

4629

NCBI Gene ID

460109

UniProt

P35749

Cellular Locus

Cytoskeleton

Host

Rabbit

Species Reactivity

Cat,Chicken,Cow,Dog,Guinea pig,Human,Pig,Rabbit,Rat

Immunogen

Recombinant full-length human MYH11 protein

Target Antigen

Smooth Muscle Myosin Heavy Chain

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

MYH11/2303R

Conjugation

Biotin

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cytoskeleton

Positive Control

Uterus or normal breast

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

205 kDa (MHC-1) and 200 kDa (MHC-2)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/908345/datasheet/bncb2303-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/908345/msds/bncb2303-500.pdf

CAS Number

9007-83-4

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