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Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) (F13A1/1448), CF594 conjugate

This antibody recognizes a protein of 83 kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/ ) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; Coagulation factor XIIIa; F13A; F13a1; Factor XIIIA; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase A chain; Transglutaminase. plasma

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

F13A1

Gene ID

2162

NCBI Gene ID

335513

UniProt

P00488

Cellular Locus

Cytoplasmic|Secreted (extracellular)

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)

Target Antigen

Factor-XIIIa

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

F13A1/1448

Conjugation

CF594

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Cancer, Immunology

Positive Control

293T Cells. Placenta.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

83 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|ELISA: 2-4 ug/mL for coating order Ab without BSA|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/902204/datasheet/bnc941448-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/902204/msds/bnc941448-500.pdf

CAS Number

9007-83-4

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