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Anti-Clathrin, Heavy Chain (CLTC/1431), CF594 conjugate

This antibody recognizes protein of 192 kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Clathrin heavy chain; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17 (CHC17 or CHC-17); CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CLTC

Gene ID

1213

NCBI Gene ID

491351

UniProt

P53675|Q00610

Cellular Locus

Cytoplasmic|Lysosomes|Vesicular

Host

Mouse

Species Reactivity

Human

Immunogen

Purified recombinant N-terminal fragment of human Clathrin Heavy Chain (exact sequence is proprietary)

Target Antigen

Clathrin, Heavy Chain

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

CLTC/1431

Conjugation

CF594

Source

Animal

Field of Research

Membrane trafficking

Positive Control

HeLa cells. Placenta or Prostate Carcinoma.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

192 kDa

Additionnal Information

For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/902171/datasheet/bnc941431-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/902171/msds/bnc941431-100.pdf

CAS Number

9007-83-4

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