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Anti-SOX2 (Transcription Factor) (SOX2/1792), CF640R conjugate

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); SOX-2; SRY (sex determining region Y) box 2; SRY related HMG box 2; Transcription factor SOX-2; ysb

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SOX2

Gene ID

6657

NCBI Gene ID

518438

UniProt

P48431

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant human SOX2 protein fragment (aa176-305) (exact sequence is proprietary)

Target Antigen

SOX2

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SOX2/1792

Conjugation

CF640R

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Stem cells, Transcription factors

Positive Control

MCF-7 cells or Cervical carcinoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

34 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|ELISA: 2-4 ug/mL for coating order Ab without BSA|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/897754/datasheet/bnc401792-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/897754/msds/bnc401792-500.pdf

CAS Number

9007-83-4

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