SCRIPT RT-qPCR GreenMaster

SCRIPT RT-qPCR GreenMaster is designed for quantitative real-time analyses of RNA templates using a green-fluorescent DNA stain that is structurally similar to SYBR® GREEN and has been specially developed for DNA analysis applications including real-time PCR (qPCR). The ready-to-use mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The 2x conc. mix contains all reagents required for RT-qPCR (except template and primers) allow fast and easy preparation with a minimum of pipetting steps. The premium quality enzymes and the optimized reaction buffer ensure superior real time PCR results. SCRIPT RT-qPCR GreenMaster is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR, the Hot Start Taq polymerase synthesizes DNA molecules that are complementary to the cDNA, thus generating a double-stranded DNA template. In the following cycle rounds, the DNA polymerase amplifies this double-stranded DNA template exponentially. In one-step RT-qPCR, all components for reverse transcription and PCR are combined in one tube so that both reactions take place one after the other without opening the tube. This offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination. The master mix already contains an optimized amount of RNase inhibitor to prevent a decrease in sensitivity due to the degradation of RNA by RNase contamination when using small amounts of templete material.