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SCRIPT Reverse Transcriptase

SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.

Product Specifications

Product Name Alternative

Reverse Transcriptase with increased thermal stability

Synonyms

Reverse Transcriptase with increased thermal stability

UNSPSC

41106300

UNSPSC Description

Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products

HS Code

35079090

eClass Number

32160502

eClass Product Group

PCR-Reagenz (Labor)

Applications

Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.

Concentration

200 units/μL

Purity

free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease

Form

Liquid

Autoclavability

false

Shipping Conditions

Shipped on gel packs

Storage Conditions

Avoid freeze/thaw cycles

Storage Temperature

Store at -20 °C

Shelf Life

12 months

Product Datasheet

https://cdn.gentaur.com/products/220/68dd094e061270361e3dca68/datasheet/pcr-505s.pdf

Product MSDS

https://cdn.gentaur.com/products/220/68dd094e061270361e3dca68/msds/pcr-505s.pdf

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