Multiplex PCR Master

Multiplex PCR Master is specially designed for the set-up of multiplex PCR reactions. It contains an optimized composition of polymerase, nucleotides, MgCl2 and stabilizing components in a specifically developed buffer system allowing the parallel amplification of a multitude of fragments in a single PCR assay. The master mix contains all reagents (except primer and template) in a 2x concentrated ready-to-use solution. The kit is recommended for use in routine PCR reactions and highly suitable for multiple target gene amplification in a single tube. The high specificity and sensitivity of the mix is achieved by a chemically inhibited hot-start polymerase. Its activity is blocked at ambient temperature preventing the extension of nonspecifically annealed primers and primer-dimer formations at low temperatures during PCR setup.