C8-Alkyne-dUTP
Product Specifications
UNSPSC
12352200
UNSPSC Description
Biochemicals
HS Code
29349990
eClass Number
32160405
eClass Product Group
Biochemikalie (Labor, Sonstige)
Applications
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice
to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Concentration
(100 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
false
Smiles
C1(=O)[nH]c(=O)n(cc1C#CCCCCC#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
