Recombinant Human IL36B

IL36B, also known as Interleukin 36 beta and IL1F8, is a cytokine belonging to the IL-1 family. It is actively secreted and is found to form a cytokine gene cluster on chromosome 2 with eight other IL1 cytokines. IL36B signals through the IL1Rrp2/IL-36R receptor on dendritic cells which then activates NFkB and MAPK signalling pathways to create a pro-inflammatory response. It stimulates the production of mature adipocytes, and IL6 and IL8 synovial fibroblasts. It also induces expression of some antimicrobial peptides and matrix metalloproteases. It is highly expressed in psoriatic plaques, as part of the IL36 signalling system is found in many epithelial barriers.

Product Specifications

CAS Number

9000-83-3

Gene Name

IL36B

Gene Aliases

FIL1 eta, Interleukin-1 eta, Interleukin-1 family member 8, Interleukin-1 homolog 2

Gene ID

27177

Accession Number

Q9NZH7

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute in sterile PBS at 100µg/mL (containing at least 0.1% human or bovine serum albumin)

Function

IL-36 beta can bind to human IL-1 Rrp2 Fc Chimera with a linear range of 0.15-5 µg/mL as determined by its binding ability in a functional ELISA

Molecular Weight

17.5

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.