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Anti-Vimentin(LN-6)

This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).

Product Specifications

Synonyms

VIM

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

VIM

Gene ID

7431

NCBI Gene ID

455493

UniProt

P08670

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Cat,Cow,Human,Mouse,Pig,Rabbit,Rat,Sheep

Immunogen

Human thymic nuclear extract

Target Antigen

Vimentin

Clonality

Monoclonal

Isotype

IgM

Clone

LN-6

Conjugation

Purified, with BSA

Source

Animal

Applications

IF (published) | IHC, frozen (published) | IHC, FFPE (verified) | IP (published) | WB (published)

Validated Applications

IF,IHC,IHC,FFPE,IP,WB

Field of Research

Cancer, Cytoskeleton

Positive Control

Jurkat cells, Sarcomas, Melanomas

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

57-60 kDa

Additionnal Information

Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1989 ) 37(9) 1363-1370. (IHC, FFPE; IHC, frozen; IF; IP) Int J Toxicol (2007) 26:289-295. (IHC; WB) Stem Cell Res Ther (2018) 9: 178 (Flow, intracellular)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/894524/datasheet/bnub0060-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/894524/msds/bnub0060-100.pdf

CAS Number

9007-83-4

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