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Anti-TGFalpha(1E8-G6)

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Product Specifications

Synonyms

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TGFA

Gene ID

7039

NCBI Gene ID

170009

UniProt

P01135

Cellular Locus

Secreted (extracellular)

Host

Mouse

Species Reactivity

Human,Rabbit,Zebrafish

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF?.

Target Antigen

TGF-Alpha

Clonality

Monoclonal

Isotype

IgM κ

Clone

1E8-G6

Conjugation

Purified, BSA-free

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Signal transduction

Positive Control

Jurkat or Ramos cells. Testicular Carcinoma

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

6 kDa

Additionnal Information

Immunohistology formalin-paraffin 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/892549/datasheet/bnum0785-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/892549/msds/bnum0785-50.pdf

CAS Number

9007-83-4

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