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Anti-pgp9.5 (UCHL-1)(UCHL1/775), Biotin conjugate

This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Gracile Axonal Dystrophy|Neuron Cytoplasmic Protein 9.5|Park5|Parkinson Disease 5|PGP95|Protein Gene Product 9.5|Ubiquitin Carboxyl-terminal Esterase L1|Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1|Ubiquitin Thioesterase L1|Ubiquitin Thiolesterase L1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

UCHL1

Gene ID

7345

NCBI Gene ID

518731

UniProt

P09936

Cellular Locus

Cytoplasmic|Endoplasmic reticulum

Host

Mouse

Species Reactivity

Human,Rat

Immunogen

Recombinant human UCHL1 protein

Target Antigen

PGP9.5 | UchL1

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

UCHL1/775

Conjugation

Biotin

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Metabolism, Neuroscience

Positive Control

Cerebellum

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

20-30 kDa

Additionnal Information

Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Western blotting 0.5-1 ug/mL|Predicted to show broad species reactivity|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/890005/datasheet/bncb0775-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/890005/msds/bncb0775-500.pdf

CAS Number

9007-83-4

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