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Anti-PCNA(PC10)

Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PCNA

Gene ID

5111

NCBI Gene ID

147433 & 728886

UniProt

P12004

Cellular Locus

Nucleus & cytoplasm

Host

Mouse

Species Reactivity

Chicken,Drosophila,Human,Monkey,Mouse,Pig,Rat,Yeaste,Yeast,Zebrafish

Immunogen

Rat PCNA/Protein A fusion protein

Target Antigen

PCNA

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PC10

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, intracellular (verified) | IHC, FFPE (verified)

Validated Applications

FC,IHC,FFPE

Field of Research

Cell cycle

Positive Control

Tonsil or reactive lymph node

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

36 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/889811/datasheet/bnum0467-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/889811/msds/bnum0467-50.pdf

CAS Number

9007-83-4

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