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Anti-p34 / cdk1(POH-1), Biotin conjugate

Recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Cdc2; CDC28A; CDK1; CDKN1; CELL CYCLE CONTROLLER CDC2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell division protein kinase 1; Cyclin-dependent kinase 1; p34 Protein Kinase; P34CDC2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CDK1

Gene ID

983

NCBI Gene ID

334562

UniProt

P06493

Cellular Locus

Nucleus & cytoplasm

Host

Mouse

Species Reactivity

Cow,Human,Mink,Monkey

Immunogen

Purified recombinant human p34cdc2 fusion protein

Target Antigen

Cdk1 | p34

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

POH-1

Conjugation

Biotin

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Cell cycle, Tumor suppressors

Positive Control

HeLa or MCF-7 Cells or Tonsil

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

34 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with mouse, rat, Xenopus, Drosophila, S. pombe, or S. cerevisiae; others not known|Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/889207/datasheet/bncb0853-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/889207/msds/bncb0853-500.pdf

CAS Number

9007-83-4

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