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Anti-Adipophilin / Perilipin-2 (Marker of Obesity) (ADFP/1493), CF740 conjugate

Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Perilipin-2; Adipophilin; ADFP; ADRP; Adipose differentiation-related protein; Perilipin-2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PLIN2

Gene ID

123

NCBI Gene ID

3416

UniProt

Q99541

Cellular Locus

Cytoplasmic

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)

Target Antigen

Adipophilin | Perilipin-2

Clonality

Monoclonal

Isotype

IgG2b λ

Clone

ADFP/1493

Conjugation

CF740

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Endocrinology, Metabolism

Positive Control

HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

48 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|ELISA For coating, order Ab without BSA|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/888754/datasheet/bnc741493-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/888754/msds/bnc741493-500.pdf

CAS Number

9007-83-4

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