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Anti-Nucleolar / Nucleoli(NM95)

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Not Known

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Cellular Locus

Nucleoli

Host

Mouse

Species Reactivity

Human

Immunogen

Nuclei of myeloid leukemia biopsy cells

Target Antigen

Nucleoli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

NM95

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Organelle markers

Positive Control

Any human cells. Tonsil

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

Not Known

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with mouse, rat or cow; others not known|Immunohistology formalin-paraffin 0.25-0.5 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunocytochemistry acetone-fixed cells 0.5-1.0 ug/mL|Immunofluorescence 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/888481/datasheet/bnum0095-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/888481/msds/bnum0095-50.pdf

CAS Number

9007-83-4

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