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Rabbit anti Human HSF-1

Product Specifications

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

CAS Number

9007-83-4

Synonyms

Heat shock factor Protein 1

UniProt

Q00613

Host

Rabbit

Species Reactivity

Human

Isotype

Ig

Conjugation

Affinity Purified

Type

Antigen Immunoaffinity Purified Polyclonal

Applications

WB, IHC

Purification Method

Antigen Immunoaffiinity Purification

Assay Principle

Antibody can be used for immunohistochemistry (2 µg/mL) and Western blotting (1-2 µg/mL). Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Jin, X., et al. ‘Targeted deletion of Hsf1, 2, and 4 genes in mice.’ Methods Mol. Biol., 787, 1-20 (2011) 2. Kaitsuka, T., et al. ‘Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing.’ EMBO Rep., 12, 673-681 (2011)

Shipping Conditions

Ambient Temperature, freeze upon arrival

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

Functional Analysis

WB

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